Botan leads Yuusuke through the Spirit World. As they are flying he looks down and sees the river Styx, as she stares straight ahead and keeps them on course.
Yuusuke is missing in this layer, but I thought it was a really nice shot of Botan. I actually saw this exact cel up for auction and missed out. But I've been lucky and have gotten a lot of second chances lately :) I love the motion in her hair, and the detail in her eyes.